P53 Status Prostate Cancer Cell Lines

One variable that may affect the radiosensitivity of prostate tumor cells is their p53 status because this gene controls radiation induced cell cycle arrest apoptosis and the repair of dna damage.

P53 status prostate cancer cell lines. Increased murine double minute 2 mdm2 expression independent of p53 status is associated with increased cancer specific mortality for men with prostate cancer treated with radiotherapy. We report an unexpected non canonical role of caspase 8 in the nucleus of cancer cells. One strain lncap gw had a mutation in one of two alleles at position 273 arg. Our laboratory has two separate culture lines of the lncap human.

External beam radiation therapy is an effective therapy for localized prostate cancer although failures occur at high rates. A paradigm for cell line cross contamination. P53 gene structure and chromosome 17p alleles were studied in the three human prostate cancer cell lines lncap du 145 and pc 3. There is growing evidence that these mutant p53s have both lost wild type p53 tumor suppressor activity and gained functions that help to contribute to malignant progression.

Although no genetic alteration is found in the p53 gene of these cell lines it is possible that. Our laboratory has two separate culture lines of the lncap human prostate cancer cells. P53 gene structure and chromosome 17p alleles were studied in the three human prostate cancer cell lines lncap du 145 and pc 3. Upon g2 m checkpoint activation caspase 8 cleaves the de ubiquitinase usp28 inhibiting its ability to stabilize p53.

This leads to a de facto p53 loss enabling cancer cells to escape p53 dependent apoptosis in favor of mitotic cell division. Analysis of p53 mutation status in human cancer cell lines. Lapc 4 p53 r175h and vcap p53 r248w cells were treated with dmso or peitc for 24 h. P53 gene structure and chromosome 17p alleles were studied in the three human prostate cancer cell lines lncap du 145 and pc 3.

Understanding the functions of mutant p53 will help in the development of new therapeutic. Four tables are presented for each type of cancer. A percent cell proliferation was determined by the wst 1 assay and b apoptosis was measured by annexin v staining by flow cytometry using a bd lsrfortessa instrument. We assessed mi 219 a small molecule inhibitor of mdm2 with improved pharmacokinetics over nutlin 3 for sensitization of prostate cancer cells to radiotherapy and androgen deprivation therapy a.

Cell lines with a wt p53 gene. One strain lncap gw had a mutation in one of two alleles at position 273 arg his. Our laboratory has two separate culture lines of the lncap human prostate cancer cells. We were highly concerned by the finding that many cell lines had controversial tp53 status including popular cell lines such as those from the nci 60 panels.

Using a system in which p53 function was conditionally restored to p53. Cell line p53 handbook v 1 0 this version of the p53 handbook includes the p53 status of 1 300 cell lines.